detection of antiseptic-resistance genes in pseudomonas and acinetobacter spp. isolated from burn patients

background: quaternary ammonium compounds (qac), which contain benzalkonium chloride as the most widely used agent, are employed as wound and skin antiseptics, as well as disinfectants in hospitals. the resistance mechanism to disinfectants is usually determine by genes which are related to resistance to quaternary ammonium compounds, namely, qace, qacδe1, qacδe1 that are found in gram-negative bacteria. objectives: the aim of this study was to determine the incidence of antiseptic resistance genes, qace and qacδe1, in clinical isolates of pseudomonas aeruginosa and acinetobacter bumanii. materials and methods: in this study, 83 clinical isolates of pseudomonas aeruginosa, and 5 isolates of acinetobacter baumannii from burn hospitals in tehran and isfahan provinces in 2010-2011, were tested by the pcr method. results: out of the 83 clinical isolates of pseudomonas aeruginosa, 49 isolates (50%) had the qace gene, and 76 isolates (91.5%) had the qacδe1 gene. in addition, in 5 isolates of acinetobacter bumanii, 2 isolates (40%) had the qace gene, and 4 isolates (80%) had the qacδe1 gene. conclusions: this study shows that the genes which harbored resistance to quaternary ammonium compound antiseptics are widespread among pseudomonas aeruginosa and acinetobacter bumanii isolates in burn patients.